OsteXcyte™ Differentiation Media

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Mesenchymal stem cells (MSCs) are functionally defined by their capacity to self renew and their ability to differentiate into multiple cell types including adipocytes, chondrocytes, and osteocytes.  differentiated osteoblasts feature vast extracellular calcium deposits in vivo and in vitro. Calcium deposits are therefore an indication of successful differentiation of MSC into osteoblasts and in vitro bone-formation. Calcium deposits can specifically be stained bright orange-red using Alizarin Red S (Figure 1).

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OsteXcyte™ Differentiation Media has been designed to induce the osteogenic differentiation of mesenchymal stem cells (MSCs) in in vitro culture. The media contains everything required for the induction of MSCs towards commitment to the osteogenesis pathway and generation of osteocytes. Using OsteXcyte™ Differentiation Media in combination with Xcyte™ MSC Xeno-Free Media provides a standardized workflow solution for MSC isolation, expansion and differentiation into calcium depositing osteocytes.

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